1.

電子ブック

EB
石金正裕, うえたに夫婦著
出版情報: 東京 : 南山堂, 2019.8
オンライン: https://elib.maruzen.co.jp/elib/html/BookDetail/Id/3000083739
2.

図書

図書
松本哲哉編著
出版情報: 大阪 : 医薬ジャーナル社, 2017.10
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3.

図書

図書
舘田一博編
出版情報: 大阪 : 医薬ジャーナル社, 2014.4
シリーズ名: インフォームドコンセントのための図説シリーズ
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4.

論文(リポジトリ)

論文(リポジトリ)
岩崎, 健 ; 鮫島, 好美 ; 下門, 奈月 ; 日越, 博信 ; Iwasaki, Ken ; Samejima, Yoshimi ; Shimojoh, Natsumi ; Higoshi, Hironobu
出版情報: 琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus.  pp.53-59,  2003-12-01.  琉球大学農学部
URL: http://hdl.handle.net/20.500.12000/3595
概要: 今回、2組のホルスタイン種親牛(A親牛およびB親牛)の分娩後1日目と7日目と、各子牛(A子牛およびB子牛)の出生後1日目から7日目までに分離した直腸便由来大腸菌252株(A子牛102株、B子牛90株、A親牛30株、B親牛30株)について、A BPC、CP、CTC、KM、SM、NA、およびSAの7薬剤に対する感受性試験を行い、耐性菌については伝達性Rプラスミドの保有状況を調査した。1)供試菌株の69%(173/252株)が薬剤耐性を示した。個体別による耐性菌検出率の平均は、A親牛20%に対してA子牛で61%、一方B親牛80%に対してB子牛90%と、いずれも親牛より子牛で高率に耐性菌が検出された。生後1日目の子牛では、A子牛47%、B子牛93%とすでに高率に耐性が認められ、生後4日目以降に耐性菌検出率がさらに増加した。2)薬剤耐性菌を薬剤別でみてみると、A親牛は4薬剤(ABPC、CTC、SM、およびSA)、B親牛は5薬剤(CP、CTC、SM、NA、およびSA)でのみ耐性菌が検出された。各子牛ではすでに1日目で親牛と同じ薬剤に耐性を示し、産後日数の経過とともに親牛では認められなかった薬剤にも耐性を示した。A子牛では2日目で他の3薬剤(CP、KM、およびNA)に、B子牛では5日目で他の1薬剤(ABPC)に耐性を示す一方、KMへの耐性は生後7日目まで認められなかった。3)薬剤耐性型をみると、A親牛で3剤型だけの2種類、B親牛で単剤型から4剤型までの5種類に対して、A子牛で単剤型から7剤型まで12種類、B子牛で単剤型から5剤型までの12種類と、子牛の薬剤耐性型が親牛よりも明らかに多かった。両子牛とも1日目で各親牛とは異なる耐性型が認められ、生後1日目から3日目までは単剤型が、4日目以降になると多剤耐性型が優勢となった。4)76株の薬剤耐性菌(A親牛6株、A子牛28株、B親牛11株、B子牛31株)について、伝達性Rプラスミドの保有状況を調査した。伝達性Rプラスミド保有菌はいずれも子牛のみから検出され、A子牛では4日目に単剤(ABPC)伝達性1株と3剤(ABPC-CP-CTC)伝達性1株、6日目に単剤(ABPC)伝達性1株が認められた。B子牛では3日目に2剤(CTC-SA)耐性型の1株だけが認められた。本調査を遂行するにあたり、御協力を頂いた沖縄県畜産試験場の各位に深謝いたします。<br />A total of 252 Escherichia coli isolates were recovered from rectal swabs of two Holstein cows (designated cow A and B) on postpartum days 1 and 7 and the female calf of each (designated calf A and B, respectively) on postnatal days 1-7. Each isolate was tested for susceptibility to 7 antibiotics [ampicillin (ABPC), chloramphenicol (CP), chlortetracycline (CTC), kanamycin (KM), streptomycin (SM), nalidixic acid (NA), and sulfadimethoxine (SA)] and for prevalence of conjugative R-plasmids. The results of these studies are: 1. Resistance to at least one of antibiotics tested was found in 69% (173/252) of the E. coli isolates. The mean frequency of resistant isolates was: cow A, 20%; calf A, 61%; cow B, 80%; and calf B, 90%. These results show that calves are readily infected with resistant bacteria: on postnatal day 1, 47% of the E. coli isolates from calf A and 93% from calf B were antibiotic-resistant. Furthermore, the fraction of isolates that were antibiotic-resistant increased after postnatal day 3. 2. On postpartum day 7, isolates with resistance to only 4 of the antibiotics tested (ABPC, CTC, SM, and SA) were obtained from cow A and to only to 5 of the antibiotics (CP, CTC, SM, NA, and SA) from cow B. On postnatal day 1, both calves already had isolates with the same-antibiotic resistances as the corresponding cow. By postnatal day 2, isolates from calf A were also resistant to the other 3 antibiotics (CP, KM, and NA). Isolates resistant to one of the other antibiotics (ABPC) were found in calf B by postnatal day 5, but no isolate to the remaining antibiotic (KM) was found in calf B by postnatal day 7. 3. A number of antibiotic resistance patterns were found, ranging from isolates resistant to a single antibiotic to isolates resistant to multiple (i. e., 2-7) antibiotics. Isolates from calves had more resistance patterns than those from cows, and no isolates from cows were resistant to more than 4 antibiotics. On postnatal day 1, isolates from both calves already had different resistance patterns than those from the corresponding cow. Isolates with resistance to a single antibiotic were predominant during postnatal day 1-3. However, after postnatal day 3, isolates with resistance to multiple antibiotics were predominant. 4. The prevalence of a conjugative R-plasmid was investigated in 76 of the antibiotic-resistant isolates; 6 from cow A, 28 from calf A, 11 from cow B, and 31 from calf B. Conjugative R plasmid transfer was found in 4 of these isolates: a day 4 postnatal isolate from calf A with resistance to 1 antibiotic (ABPC), a day 6 postnatal isolate from calf A with resistance to 1 antibiotic (ABPC), a day 4 postnatal isolate from calf A with resistance to 3 antibiotics (ABPC-CP-CTC), and a day 3 postnatal isolate from calf B with resistance to 2 antibiotics (CTC-SA). 続きを見る
5.

論文(リポジトリ)

論文(リポジトリ)
日越, 博信 ; 田中, こころ ; 田中, 志津香 ; 平川, 守彦 ; Higoshi, Hironobu ; Tanaka, Kokoro ; TaNaka, Shizuka ; Hirakawa, Morihiko
出版情報: 琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus.  pp.29-35,  1999-12-01.  琉球大学農学部
URL: http://hdl.handle.net/20.500.12000/3661
概要: 動物病院で診療を受けた,受診犬(27頭)及び受診猫(13頭),動物愛護センターで引き取られるか,捕獲された犬(20頭)及び猫(24頭),計84頭から分離された大腸菌776株について,ABPC,CP,CTC,KM,SM,NA,SAの7薬剤に対 する感受性試験を行った。また耐性菌については,伝達性Rプラスミドの検索を行った。7薬剤のいずれかに耐性であった大腸菌は,犬では102株(24.7%),猫では59株(16.3%)であった。犬の耐性菌検出率では,受診犬(24.0%)と捕獲犬(25.5%)がほぼ同率であったが,猫では受診猫26.8%に対し,引取猫10.6%で,前者が高率であった。薬剤別の検出率は,犬ではCTC耐性が17.7%で最高,以下SA,SM,ABPC耐性の順であり,猫ではSA耐性が12.6%で最高,以下ABPC,CTC,SM耐性の順であった。捕獲犬は受診犬に比較して,すべての薬剤において検出率が高かったが,猫では逆にすべての薬剤で受診猫の方が引取猫より高かった。耐性型は,犬では単剤型から6剤型まで23種類(捕獲犬22種類,受診犬10種類),猫では単剤型から7剤型まで9種類(引取猫6種類,受診猫4種類)であった。受診犬は単剤型と2剤型で73%を占めたが,捕獲犬では3剤型以上が60%に達した。猫では,引取猫の過半数が単剤型と2剤型であったのに対し,受診猫は7剤型のみで62%を占めた。耐性菌における伝達性Rプラスミド保有菌の検出率は,犬が6.1%,猫が35.7%であった。猫では殆どが2剤伝達性であったが,犬では単剤伝達性が殆どであった。<br />Tests for sensitivity to seven drugs were conducted on 776 strains of Escherichia coli isolated from a total of 84 dogs and cats; 27 dogs and 13 cats referred to an animal hospital for treatment (referred group) and 20 dogs and 24 cats taken over or captured by animal protection centers (captured group). In the resistant strains, transferable R plasmid were studied. Resistance to at least one of the 7 drugs was found in 102 (24.7%) of strains obtained from dogs and 59 (16.3%) of strains obtained from cats. The percentage of resistant strains was 24.0% from referred dogs and 25.5% from captured dogs. The corresponding values were 26.8% in referred cats and 10.6% in captured cats. In dogs, the rate of resistance was the highest for CTC (17.7%) followed by SA, SM and ABPC in decreasing order. In cats, the rate of resistance was the highest for SA (12.6%) followed by ABPC, CTC and SM. The rate of drug resistance was higher for each drug in captured dogs than in referred dogs, but higher for each drug in referred cats than in captured cats. As to the range of multiple resistance, 23 combinations ranging from resistance to a single drug to 6 drugs were found in dogs (22 combinations in captured dogs and 10 combinations in referred dogs), whereas 9 combinations ranging from resistance to a single drug to all 7 drugs were found in cats (6 combinations in captured cats and 4 in referred cats). In referred dogs, resistance to 1 or 2 drugs accounted for 73%, whereas resistance to 3 or more drugs accounted for 60% in captured dogs. More than half of the strains from captured cats showed resistance to only 1 or 2 drugs, whereas strains resistant to all 7 drugs accounted for 62% from referred cats. Resistant strains with transferable R plasmid were found in 6.1% of dogs and 35.7% of cats. While 2 drug transfer was mostly found in cats, single drug transfer was mostly found in dogs. 続きを見る
6.

図書

図書
吉川昌之介著
出版情報: 東京 : 中央公論社, 1995.3
シリーズ名: 中公新書 ; 1234
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7.

論文(リポジトリ)

論文(リポジトリ)
日越, 博信 ; 山城, 倫子 ; 長田, 健司 ; Higoshi, Hironobu ; Yamashiro, Rinko ; Nagata, Kenji
出版情報: 琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus.  pp.147-153,  1994-12-01.  琉球大学農学部
URL: http://hdl.handle.net/20.500.12000/3760
概要: 食肉における薬剤耐性大腸菌の汚染状況を知る目的で, 沖縄県内の某と畜場でと殺解体直後の豚枝肉について調査を行った。試料は, 解体直後の6と体の豚枝肉から, 処理工程別に背割後, 洗浄後, 格付け前, 冷蔵庫内の4ヵ所で拭き取り法によって採取 した。また, 湯浸け水(脱毛のためにと体を浸漬する湯), 内臓液(内臓摘出時に出てくる液)についても調査した。これらの試料から合計301株の大腸菌を分離し, 7薬剤に対する感受性試験を行った。供試した大腸菌301株のうち195株(64.8%)が, いずれか1薬剤に耐性であった。枝肉処理の工程別にみた耐性菌の検出率は, 背割後74.1%, 洗浄後53.4%, 格付け前57.9%, 冷蔵庫内65.6%であった。内臓液由来では91.7%が耐性であったが, 湯浸け水からは大腸菌は分離されなかった。薬剤別には, CTC耐性が51.5%, SA耐性が35.5%, SM耐性が29.2%などの順に高く, これら3薬剤に対する耐性菌の検出順位は各試料とも同じであった。耐性型は, 単剤型から7剤型まで46種類認められ, その種類は処理工程が進むほど減少した。また全体では単剤型が29.7%, 3剤型が18.5%, 2剤型が17.4%, 4剤型が16.9%の順であったが, 2剤以上の薬剤に耐性の多剤耐性菌は, 背割後由来で検出率が最も高かった。耐性菌170株のうち9株(5.3%)がRプラスミド保有菌であり, 少なかった。<br />Immediate post-slaughtering contamination of pork carcasses with drug-resistant Escherichia coli was investigated in a slaughter house in Okinawa Prefecture. A total of 301 strains of E. coli were isolated from dressed pork carcasses. Drug sensitivity testing of these strains to seven antibiotics was performed. Among the 301 strains of E. coli tested, 195 strains (64.8%) exhibited drug resistance. Isolation rates of drug-resistant strains at various stages in processing were 74.1% after halving, 53.4% after cleaning, 57.9% befor grading and 65.6% in refrigeration. Strains isolated from visceral fluid samples were 91.7% drug-resistant. No E. coli was isolated from the hot water used for dehairing. The highest resistance found among the antibiotics tested was to CTC (51.5% of strains), SA (35.5%) and SM (29.2%). This same order of resistance was found in all samples. Forty six patterns of resistance were observed, ranging from single-agent to seven-agent resistance. The number of patterns of resistance decreased as the carcasses advanced through processing. For all strains isolated, 29.7% were resistant to one agent, 17.4% to two agents, 18.5% to three agents and 16.9% to four agents. Strains resistant to more than two agents were most commonly isolated from carcasses after halving. Only nine strains (5.3%) among the 170 drug-resistant strains possessed R plasmids. 続きを見る
8.

論文(リポジトリ)

論文(リポジトリ)
日越, 博信 ; 長田, 健司 ; 山城, 倫子 ; 平川, 守彦 ; Higoshi, Hironobu ; Nagata, Kenji ; Yamashiro, Rinko ; Hirakawa, Morihiko
出版情報: 琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus.  pp.155-160,  1994-12-01.  琉球大学農学部
URL: http://hdl.handle.net/20.500.12000/3761
概要: 沖縄県における家畜糞便由来薬剤耐性大腸菌の検出状況の調査の一環として, 今回はと畜場で解体直後のウシの盲腸内容物由来について調査を行った。ウシの盲腸内容物試料は, 品種等別に外産牛20頭, 黒毛和種5頭, ホルスタイン種5頭, 計30頭から 採取し, 黒毛和種については農場で肥育中のウシ10頭の糞便も採取した。これらの試料から合計343株の大腸菌を分離し, 7薬剤に対する薬剤感受性試験を行った。供試した大腸菌343株のうち45株がいずれか1薬剤に耐性であり, その検出率は13.1%と低率であった。ウシの品種等別では, 外産牛が18.8%, 黒毛和種が6.8%, ホルスタイン種が6.7%であり, 外産牛で高かった。薬剤別の耐性菌は, 全体ではSA耐性24株, CTC耐性20株, KM耐性14株などが比較的多かった。外産牛ではSA及びCTC耐性が多く, またホルスタイン種では各薬剤に対する耐性菌が検出されたが, 黒毛和種ではKM及びSA耐性のみであった。耐性型は, 全体では12種類認められたが, 外産牛では単剤型から4剤型まで9種類, 黒毛和種では単剤型のみ2種類, ホルスタイン種では6剤型と7剤型の2種類であつた。耐性菌におけるRプラスミド保有菌は4株あり, すべて外産牛由来であった。<br />As part of the study of the incidence of drug resistance of Escherichia coli of fecal origin in liverstock, the drug resistance of E. coli isolated from the cecal contents of cattle was studied. Cecal contents were collected at a slaughter house in Okinawa Prefecture during the slaughtering and dressing of 30 beef cattle including 20 imported cattle (imported, then fattened in the Prefecture), five Japanese blacks and five Holsteins. Fecal samples were also collected from 10 Japanese blacks being fattened on a farm. A total of 343 strains of E. coli were isolated from these samples. Drug sensitivity testing of these strains to seven antibiotics was performed. Of the 343 strains, 45 were resistant to at least one antibiotic, with a low isolation rate of 13.1%. The isolation rate of drug-resistant strains was 18.8% in imported cattle, 6.8% in Japanese blacks and 6.7% in Holsteins. The highest rate was found in imported cattle. Resistance to SA, CTC and KM was relatively high, with 24 strains resistant to SA, 20 to CTC and 14 to KM. Many SA-and CTC-resistant strains were isolated from the imported cattle. Strains resistant to all antibiotics tested were isolated from the Holsteins, while only KM- or SA-resistant strains were isolated from the Japanese blacks. Twelve different patterns of resistance were observed in all the strains. Nine patterns of resistance, including one- to four-agent resistance, were detected in imported cattle. Two patterns of single-agent resistance were found in Japanese blacks and two patterns of six-and seven-agent resistance were found in Holsteins. Attempts were made to detect R plasmid in the resistant strains. It was detected in only 4 strains, all isolated from imported cattle. 続きを見る
9.

図書

図書
鶴尾隆編
出版情報: 東京 : 羊土社, 1994.10
シリーズ名: Newメディカルサイエンス
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10.

図書

図書
林康之, 猪狩淳編集企画
出版情報: 東京 : 金原出版, 1987.8
シリーズ名: 臨床検査MOOK / 山中學 [ほか] 編集主幹 ; No.25
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