核DNAマーカーによるミナミメダカ沖縄諸島タイプの 確率的判定と低い遺伝的多様性

資料種別:
論文(リポジトリ)
責任表示:
畑中, ひらり ; 今井, 秀行 ; Hatanaka, Hirari ; Imai, Hideyuki
言語:
日本語
出版情報:
日本生物地理学会 — The Biogeographical Society of Japan, 2018-12-20
著者名:
掲載情報:
日本生物地理学会会報 = Bulletin of the Bio-geographical Society of Japan
ISSN:
0067-8716  CiNii Articles  Webcat Plus  JAIRO
通号:
73
開始ページ:
60
終了ページ:
69
バージョン:
publisher
概要:
This study included 508 individuals of Oryzias latipes from 15 sites in mainland Japan and 10 sites in the Ryukyu Archipelago and Taiwan used in Imai et al. (2017), and new samples from four sites in the Ryukyu Archipelago, four sites in China and South Korea, and one from hi-medaka. We developed four new PCR-RFLP nuclear DNA markers (Orla16- 185-MboI, Orla1-29-BstUI, Orla1-51-AluI, and P0Ex-HinfI) based on three universal microsatellite primer sets and an exon-primed intron-crossing (EPIC) primer set for fish. STRUCTURE analysis indicated that Amami-oshima, Iheya, Izena, Ginama, Ogimi, and Bios no Oka formed a cluster, while specimens from Fukuchi, the former Kadena Ammunition Storage area, Zamani, and other Okinawa Islands formed a different cluster. The genetic diversity of subpopulations from Fukuchi, the former Kadena Ammunition Storage area, and Zamani was higher than those from other Okinawa Islands. The individual exclusion test (>90% condition) was applied to the Okinawa Islands subpopulation using GeneClass2. It will be important to apply these genetic markers for periodical genetic monitoring of this subpopulation. 続きを見る
URL:
http://hdl.handle.net/20.500.12000/43516
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