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| 1.
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Shotaro, Nakanishi ; Miyazato, Minoru ; Tanaka, Kei ; Uema, Namiko ; Saito, Seiichi
概要:
Congenital mid-ureteral strictures (CMS) are rare. Most congenital strictures occur at the ureteropelvic junction or ure
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terovesical junction, with mid-ureteral strictures accounting for only 4–5% of all cases of ureteral obstruction in children. Furthermore, there are very few reports of coexisting mid-ureteral stricture and ureterovesical junction obstruction (UVJO). Here, we report a case of coexisting UVJO and CMS. CMS was not detected on preoperative magnetic resonance imaging, and hydronephrosis remained after ureteroneocystostomy. Therefore, MRI was repeated and CMS was diagnosed, for which we performed ureteroureterostomy. Intraoperative retrograde pyelography (RGP) aids definitive diagnosis of UVJO.
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| 2.
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Koikea, Shin ; Nakanishi, Shotaro ; Nohara, Sunao ; Miyahira, Hirofumi ; Tamaki, Tomoko ; Saito, Seiichi
概要:
Large adrenocortical adenomas have rarely been reported. We describe a case of a 26-year-old man who underwent an adrena
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lectomy for a large adrenocortical adenoma (8.6 × 7.7 cm). Although the lesion had typical malignant features on imaging, histopathological examination revealed an adrenocortical adenoma. This highlights that imaging alone may not be able to distinguish adrenocortical carcinomas from adrenal masses. In most cases, a resection should be performed for early diagnosis and management of large adrenal masses with malignant features on imaging. To our knowledge, this is the first report of a large adrenocortical adenoma diagnosed with multiple imaging investigations.
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| 3.
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Tsuzuki, Shunsuke ; Nakanishi, Shotaro ; Tamaki, Mitsuyoshi ; Oshiro, Takuma ; Miki, Jun ; Yamada, Hiroki ; Shimomura, Tatsuya ; Kimura, Takahiro ; Furuta, Nozomu ; Saito, Seiichi ; Egawa, Shin
概要:
Background:There was no clear evidence whether the initial dose of enzalutamide affects the incidence of adverse events
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(AEs), and oncological outcome in patients with castration-resistant prostate cancer (CRPC).\nMethods:The clinical charts of 233 patients with CRPC treated with enzalutamide were reviewed retrospectively. After 1:3 propensity score matching (PSM), 124 patients were divided into a reduced dose group and a standard dose group, and the prostate specific antigen (PSA) response and the incidence of AEs were compared.\nResults:190 patients with CRPC initiated with standard dose enzalutamide were younger and better performance status compared with 43 patients beginning with reduced dose. After PSM, the baseline characteristics were not different between the standard and the reduced dose group. In the PSM cohort, the PSA response rate was significantly lower in the reduced dose group than in the standard dose group (-66.3% and -87.4%, p = 0.02). The incidence rates of AEs were not statistically different between the groups (22.6% and 34.4%, respectively, p = 0.24).\nConclusion:\nInitiating treatment with a reduced dose of enzalutamide did not significantly decrease the incidence rate of AEs, and it showed poorer PSA response rate. There is no clear rationale for treating with a reduced initial dose of enzalutamide to reduce the incidence of AEs.
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| 4.
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Nakanishi, Shotaro ; Goya, Masato ; Tamaki, Mitsuyoshi ; Oshiro, Takuma ; Saito, Seiichi
概要:
Objective:To date, there are no useful markers for predicting the prognosis of metastatic hormone-sensitive prostate can
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cer (mHSPC). We evaluated the effect of early changes in prostate-specific antigen (PSA) levels after androgen deprivation therapy (ADT) on castration-resistant prostate cancer (CRPC) progression and overall survival (OS) in mHSPC patients.\nResults:In 71 primary mHSPC patients treated with ADT, the median times to CRPC and OS were 15 months and 92 months, respectively. In multivariate analysis, a Gleason score of ≥ 8 (p = 0.004), an extent of disease value (EOD) of ≥ 2 (p = 0.004), and a 3-month PSA level > 1% of the pretreatment level (p = 0.017) were independent predictors of shorter time to CRPC. The area under the receiver operating characteristic curve was feasible at 0.822. A 3-month PSA level > 1% of the pretreatment level was an independent predictor of OS (p = 0.004). Three factors were independent predictors of shorter time to CRPC. A 3-month PSA level > 1% of the pretreatment level correlated with a poor prognosis.
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| 5.
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Miyazato, Minoru ; Ashikari, Asuka ; Nakamura, Koshi ; Nakamura, Takehiro ; Yamashiro, Kiyoto ; Uema, Tsugumi ; Uehara, Moriyuki ; Masuzaki, Hiroaki ; Saito, Seiichi ; Maeda, Shiro ; Ishida, Hajime ; Matsushita, Masayuki
概要:
Purpose:To evaluate the effect of a mobile digital intervention on voiding patterns, we performed 24-h voided volume mon
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itoring in individuals with metabolic disorders.\nMethods:Participants with metabolic disorders were grouped into either the intervention group (n = 17), who had access to a smartphone app (CARADA), or the non-intervention group (n = 11), who did not. Urine monitoring was conducted for 24 h using a novel digital self-health monitoring system for urine excretion (s-HMSU). Body weight, abdominal circumference, blood pressure, and biomarkers were measured.\nResults:Physical findings and blood test results at baseline and 6 months indicated no significant between-group differences. Night-time frequency did not change between baseline and 6 months in the intervention group but significantly worsened at 6 months in the non-intervention group, as compared to baseline (1.0 ± 0.7 vs. 1.5 ± 0.5, p < 0.05). The change in night-time frequency over 6 months did not differ between the intervention and non-intervention groups. Furthermore, the change in hours of undisturbed sleep over 6 months did not differ between the two groups. However, compared with baseline, nocturnal polyuria index tended to worsen at 6 months in the non-intervention group.\nConclusion:Our study results suggest that mobile digital intervention might be useful for behavioral therapy to improve night-time frequency and urine production and that s-HMSU might be beneficial for confirming the prevention of progress in individuals with metabolic disorders, which can aid in modifying lifestyle.
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| 6.
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Tsuchida, Akiko ; Senda, Motohiro ; Ito, Akihiro ; Saito, Seiichi ; Kiso, Makoto ; Ando, Takayuki ; Harduin-Lepers, Anne ; Matsuda, Akio ; Furukawa, Keiko ; Furukawa, Koichi
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| 7.
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Nakanishi, Shotaro ; Nishida, Sho ; Miyazato, Minoru ; Goya, Masato ; Saito, Seiichi
概要:
We present a 78-year-old male with renal cell carcinoma who developed myasthenia gravis complicated by myositis after ni
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volumab administration, which was verified by the presence of antibodies against the acetylcholine receptor. The initial symptom was posterior neck pain, and biochemical examination of blood showed elevated levels of hepatic enzymes and creatine phosphokinase. The level of antibody against the acetylcholine receptor increased 4.1-fold. His condition progressed rapidly resulting in respiratory failure 15 days after conservative therapy.
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| 8.
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Nakanishi, Shotaro ; Nishida, Sho ; Miyazato, Minoru ; Goya, Masato ; Saito, Seiichi
概要:
We present a 78-year-old male with renal cell carcinoma who developed myasthenia gravis complicated by myositis after ni
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volumab administration, which was verified by the presence of antibodies against the acetylcholine receptor. The initial symptom was posterior neck pain, and biochemical examination of blood showed elevated levels of hepatic enzymes and creatine phosphokinase. The level of antibody against the acetylcholine receptor increased 4.1-fold. His condition progressed rapidly resulting in respiratory failure 15 days after conservative therapy.
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| 9.
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Yonamine, Tomoko ; Kaname, Tadashi ; Chinen, Yasutsugu ; Tamashiro, Kouichi ; Kosuge, Noritake ; Saito, Seiichi
概要:
Hereditary leiomyomatosis and renal cell cancer is a rare, inherited disease caused by mutations in the fumarate hydrata
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se gene. It is characterized by cutaneous leiomyomas, uterine leiomyomas, and/or renal cell cancer. We present the case of a 42-year-old woman with a heterozygous missense mutation (p.M195T) in the fumarate hydratase gene. Although the patient did not have cutaneous leiomyoma and she had no family history of hereditary leiomyomatosis and renal cell cancer, the presence of early onset symptomatic uterine leiomyoma and type 2 papillary renal cell cancer confirmed the diagnosis of hereditary leiomyomatosis and renal cell cancer.
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| 10.
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Matsumura, Eri ; Kosuge, Noritake ; Nakanishi, Shotaro ; Suda, Tetsuji ; Sugawa, Ai ; Fujimura, Tsutomu ; Miyagi, Ryota ; Yoshimi, Naoki ; Saito, Seiichi
概要:
Urothelial carcinoma of the bladder (UCB) is potentially life-threatening; therefore, we aimed to discover a novel urine
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biomarker for diagnosis and prognostication of UCB. This is a retrospective case-control study. Exploration of a new biomarker using urine from 20 UCB patients in the present study revealed that urinary level of lactoferrin (LF), a multifunctional glycoprotein released from neutrophils, was higher in 11 of 15 with invasive/high-grade UCB than 5 with non-invasive one, and 2 healthy adults. We therefore focused on LF and assessed the value of urine LF normalized by urine creatinine concentration (LF/Cr) using an enzyme-linked immunosorbent assay. Diagnostic performance of urine LF/Cr was examined using urine from 92 patients with primary (newly diagnosed) untreated UCB and 166 controls without UCB, including 62 patients with pyuria, and 104 subjects without pyuria consisting of 84 patients and 20 healthy adults. However, the diagnostic accuracies were accompanied by the risk of bias. In 92 primary UCB patients, both pyuria and tumor-infiltrating neutrophils (TINs) were independent predictors for urine LF/Cr. In contrast, TINs or urine LF/Cr were independent predictors for invasive histology, whereas pyuria was not. In terms of prognostication, urine LF/Cr and nodal metastasis were independent predictors of disease-specific survival in 22 patients with muscle-invasive bladder cancer, characterized by a high mortality rate, in the Cox proportional hazards model. In conclusion, urine LF/Cr linked to TINs was a predictor of both invasive histology and prognosis in UCB. Urine LF/Cr is a potential biomarker reflecting the degree of malignancy in UCB.
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| 11.
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Nakamura, Yuichiro ; Miyata, Yasuyoshi ; Matsuo, Tomohiro ; Shida, Yohei ; Hakariya, Tomoaki ; Ohba, Kojiro ; Taima, Takenobu ; Ito, Akihiro ; Suda, Tetsuji ; Hakomori, Sen-itiroh ; Saito, Seiichi ; Sakai, Hideki
概要:
Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in th
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e malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens obtained by biopsy and radical prostatectomy (RP), respectively. The relationships between SSEA-4 expression in cancer cells or the presence of SSEA-4-positive tumor-infiltrating immune cells (TICs) and clinicopathological parameters were analyzed. SSEA-4 expression in cancer cells was significantly associated with Gleason score, local progression, and lymph node and distant metastasis. In RP specimens, high SSEA-4 expression in cancer cells and the presence of SSEA-4-positive TICs were significant predictors of pT3, i.e., invasion and worse biochemical recurrence (BCR) after RP, respectively, in univariate analysis. In contrast, combination of high SSEA-4 expression in cancer cells and the presence of SSEA-4-positive TICs was an independent predictor for pT3 and BCR in multivariate analysis. Biologically this combination was also independently associated with suppression of apoptosis. Thus, the co-expression of SSEA-4 in cancer cells and TICs may have crucial roles in the malignant aggressiveness and prognosis of PC. Invasive potential and suppression of apoptosis may be linked to SSEA-4 expression.
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| 12.
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Tsuchida, Akio ; Senda, Motohiro ; Ito, Akihiro ; Saito, Seiichi ; Kiso, Makoto ; Ando, Takayuki ; Harduin-Lepers, Anne ; Matsuda, Akio ; Furukawa, Keiko ; Furukawa, Koichi
概要:
GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell
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cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.
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| 13.
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Nakanishi, Shotaro ; Miyazato, Minoru ; Miyagi, Ryota ; Saito, Seiichi
概要:
A male newborn was referred to our institute for postrenal acute renal failure with bilateral hydroureteronephrosis, ele
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ctrolyte imbalance, and anuria. Voiding cystourethrography revealed no vesicoureteral reflux or posterior urethral valves. We performed bilateral percutaneous nephrostomy on the 9th post-natal day. The serum creatinine level improved to the normal range. Antegrade pyelography revealed bilateral uretero-vesical junction stenosis. Subsequently, we inserted bilateral double J urethral stents at 1 month. Bilateral ureteroneocystostomy without ureteral folding was performed at 6 months. The postoperative course was uneventful. This is the second case of postrenal acute renal failure with bilateral primary obstructive megaureters in a neonate.
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| 14.
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Maruyama, Nobuyuki ; Nakasone, Toshiyuki ; Arakaki, Osao ; Matsumoto, Hirofumi ; Maruyama, Tessho ; Matayoshi, Akira ; Goto, Takahiro ; Saito, Seiichi ; Yoshimi, Naoki ; Arasaki, Akira ; Nishihara, Kazuhide
概要:
Second primary cancer (SPC) is an important prognostic factor for patients with head and neck cancer (HNC); therefore, t
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he association between the prognosis and development of SPC has been well‑reported. The use of 2‑[18F]‑fluoro‑2‑deoxy‑D‑glucose‑positron emission tomography (FDG‑PET) is valuable to examine cancer stage, evaluate treatment responses and investigate suspected relapses or metastases. In the present study, the case of a male patient who was diagnosed with three primary cancer types, including well to moderately differentiated squamous cell carcinoma (SCC) of the mandible, axillary cutaneous poorly differentiated SCC and prostate adenocarcinoma, was described. Among these, mandible cancer was the first diagnosed when the patient was 70 years of age. Synchronous skin and prostate cancer (PRC) types then developed 3 years later. To the best of our knowledge, this is the first report of the aforementioned combination of cancer types. Postoperative FDG‑PET was not performed as no lesions of recurrence or metastases of mandible cancer were found. Three years later, the PRC was asymptomatic and was incidentally detected by FDG‑PET performed for a preoperative evaluation of skin cancer. It was indicated that FDG‑PET could be utilized in patients with HNC due to there being no accurate FDG‑PET protocol to detect SPC over a long‑term follow‑up.
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| 15.
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大城, 吉則 ; 安次嶺, 聡 ; 木村, 隆 ; 宮城, 亮太 ; 玉城, 由光 ; 仲西, 昌太郎 ; 松村, 英理 ; 大城, 琢磨 ; 宮里, 実 ; 呉屋, 真人 ; 斎藤, 誠一 ; Oshiro, Yoshinori ; Ashimine, Satoshi ; Kimura, Ryu ; Miyagi, Ryota ; Tamaki, Mitsuyoshi ; Nakanishi, Syotarou ; Matsumura, Eiri ; Oshiro, Takuma ; Goya, Masato ; Miyazato, Minoru ; Saito, Seiichi
概要:
【Objective】Good long-term kidney graft function and graft survival depend on multiple factors. This study aimed to asses
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s the impact of donor and recipient clinical factors on 20-year graft survival after kidney transplantation (KT).\n【Material and Methods】From 1987-1994, twenty-five KTs (14 living and 11 deceased donors) were performed at Ryukyu University Hospital, and 24 of these cases were followed for more than 20 years after the KTs and reviewed. Ten patients had 20 years of graft survival, and 14 experienced graft failure for some reason within 20 years after KTs. Retrospective analyses were performed to elucidate the difference in donor and recipient factors among these patients.\n【Results】The death censored graft survival rates at 5, 10, 15, and 20 years were 92.0%, 83.0%, 67.0%, and 67.0% in living-donor KTs and 73.0%, 62.0%, 62.0%, and 31.0% in deceased-donor KTs, respectively. The overall graft survival rates at 5, 10, 15, and 20 years were 85.0%, 77.0%, 62.0%, and 54.0% in living-donor KTs and 63.0%, 55.0%, 45.0%, and 27.0% in deceased-donor KTs, respectively. Comparison of clinical factors between the ≥ 20-year graft survivor group (10 cases) and <20-year graft survivor group (14 cases) revealed no differences in, for example, recipient age, duration of dialysis, donor age, donor type, number of human leukocyte antigen mismatches, and occurrence of acute rejections within 1 year of KTs. However, among 13 cases of living-donor KTs, the overall graft survivals after KTs from donors older than 50 years were worse than those after KTs from donors younger than 50 years. Also, the death-censored graft survivals after KTs from donors older than 60 years were worse than those after KTs from donors younger than 60 years. Moreover, graft survivals after KTs from maternal donors were worse than those after KTs from nonmaternal living donors, and there were no 20-year graft survivors after KTs from maternal donors.\n【Conclusion】Our results suggest that the principal risk factors associated with <20-year graft survival after living-donor KTs are the presence of donors older than 50 or 60 years and a maternal donor.
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| 16.
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Miyazato, Minoru ; Yamashiro, Satoshi ; Goya, Masato ; Inafuku, Hitoshi ; Ikehara, Akashi ; Oshiro, Yoshinori ; Saito, Seiichi ; Kuniyoshi, Yukio
概要:
Background: Renal cell carcinoma with tumor thrombus extension into the inferior vena cava occurs in approximately 5% of
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cases. Despite such situations, an aggressive surgical approach is recommended. However, intraoperative prevention of pulmonary embolism by a fragmended tumor thrombus is necessary. To prevent pulmonary embolism, placement of a temporary suprarenal filter has been attempted, however, the precise placement of a temporary filter between the level of the hepatic vein and right atrium is not always easy because of its migration, tilting, and strut fracture. Here we report a method for early occlusion control of the intrapericardial inferior vena cava to prevent pulmonary embolism during nephrectomy in level II or III renal cell carcinoma tumor thrombus.\nCase presentation: Our first case was a 37-year-old Japanese man with left renal cell carcinoma extending into the inferior vena cava below the main hepatic vein (level II) and our second was a 75-year-old Japanese man with right renal cell carcinoma extending into the retrohepatic inferior vena cava at the main hepatic vein (level III). En block resection of the kidney and the tumor thrombus was performed with the aid of partial extracorporeal circulation; the postoperative course of both patients was uneventful.\nConclusion: Control of intrapericardial inferior vena cava is a feasible method to prevent pulmonary embolism.
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| 17.
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Miyazato, Minoru ; Yamashiro, Satoshi ; Goya, Masato ; Inafuku, Hitoshi ; Ikehara, Akashi ; Oshiro, Yoshinori ; Saito, Seiichi ; Kuniyoshi, Yukio
概要:
Background: Renal cell carcinoma with tumor thrombus extension into the inferior vena cava occurs in approximately 5% of
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cases. Despite such situations, an aggressive surgical approach is recommended. However, intraoperative prevention of pulmonary embolism by a fragmended tumor thrombus is necessary. To prevent pulmonary embolism, placement of a temporary suprarenal filter has been attempted, however, the precise placement of a temporary filter between the level of the hepatic vein and right atrium is not always easy because of its migration, tilting, and strut fracture. Here we report a method for early occlusion control of the intrapericardial inferior vena cava to prevent pulmonary embolism during nephrectomy in level II or III renal cell carcinoma tumor thrombus.\nCase presentation: Our first case was a 37-year-old Japanese man with left renal cell carcinoma extending into the inferior vena cava below the main hepatic vein (level II) and our second was a 75-year-old Japanese man with right renal cell carcinoma extending into the retrohepatic inferior vena cava at the main hepatic vein (level III). En block resection of the kidney and the tumor thrombus was performed with the aid of partial extracorporeal circulation; the postoperative course of both patients was uneventful.\nConclusion: Control of intrapericardial inferior vena cava is a feasible method to prevent pulmonary embolism.
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| 18.
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斎藤, 誠一 ; Saito, Seiichi
概要:
Prostate-specific antigen (PSA) has been widely used for detection and diagnosis of early prostate cancer. However, PSA
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has problems with specificity and does not reflect grade of malignancy. In addition, PSA has no cut-off value because the prevalence of prostate cancer was reported to be 15% among men with PSA 4 ng/mL or less. Therefore, a new serum marker to compensate for the problems of PSA is urgently required. In an attempt to search a novel marker, we focused on the glycosyl epitope, $ \beta $1, 4-GalNAcDSLc_4 , to which monoclonal antibody (mAb) RM2 was established using renal cell carcinoma cell line, based on the assumption that the hybrid structure of the glycosyl epotope is associated with the nature of prostate cancer. By immunohistochemistry using radical prostatectomy specimens,we found RM2 reacted to prostate cancer cells, reflecting grade of malignancy, whereas RM2 had no reactivity to benign prostatic hyperplasia. RM2 showed preferential reactivity toward haptoglobin-beta chain derived from prostate cancer than that from benign prostatic diseases. Haptoglobin-beta chain defined by RM2 (Hp-beta-RM2) in serum had sensitivity of 87% and specificity of 84% for detecting early prostate cancer among men with PSA value < 10 ng/mL. Hp-beta-RM2 is a novel serum marker that may be useful for detection of early prostate cancer. Furthermore, serum level of Hp-beta-RM2 may predict PSA failure after radical prostatectomy, although the number of patients examined is small.
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| 19.
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Ito, Akihiro ; Levery, Steven B. ; Saito, Seiichi ; Satoh, Makoto ; Hakomori, Sen-itiroh
概要:
In renal cell carcinoma (RCC), the level of higher gangliosides is correlated with degree of metastatic potential, and c
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ell lines derived from metastatic deposits of RCC are characterized by high expression of disialogangliosides (Saito, S., Orikasa, S., Ohyama, C., Satoh, M., and Fukushi, Y. (1991) Int. J. Cancer 49, 329–334 and Saito, S., Orikasa, S., Satoh, M., Ohyama, C., Ito, A., and Takahashi, T. (1997) Jpn. J. Cancer Res. (Gann) 88, 652–659). We now report two disialogangliosides, G1 and G2, found in the RCC cell line TOS-1. G1 from TOS-1 cells was characterized as having a novel hybrid structure between ganglio-series (region I as in Structure FTI; same as the terminal structure of ganglioside GM2), and the lacto-series type 1 (region II). The characterization was based on reactivity with various monoclonal antibodies (mAbs) with defined epitope specificity, as well as monosaccharide and fatty acid component analysis, ^1H NMR spectroscopy, and electrospray ionization mass spectrometry of the intact compound. G1 showed strong reactivity with mAb RM2, raised originally against TOS-1 cells, and weak cross-reactivity with anti-GM2 mAb MK-1–8. The antigen is hereby termed GalNAc disialosyl Lc_4Cer (IV^4GalNAcIV^3NeuAcIII^6NeuAcLc_4; abbreviated GalNAcDSLc_4). G2 was identified by^1H NMR and mass spectrometry as having a structure similar to Structure FTI but without the GalNAcβ1→4 substitution and showed strong reactivity with mAb FH9 reported previously to be specific for disialosyl lacto-series type 1 (disialosyl Lc_4) having vicinal α2→3 and α2→6 sialosyl residues, an antigen associated with human colonic cancer. Clinicopathological studies indicate that expression of these disialoganglioside antigens in RCC tissue is correlated with the metastatic potential of RCC.
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| 20.
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Ito, Akihiro ; Levery, Steven B. ; Saito, Seiichi ; Satoh, Makoto ; Hakomori, Sen-itiroh
概要:
In renal cell carcinoma (RCC), the level of higher gangliosides is correlated with degree of metastatic potential, and c
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ell lines derived from metastatic deposits of RCC are characterized by high expression of disialogangliosides (Saito, S., Orikasa, S., Ohyama, C., Satoh, M., and Fukushi, Y. (1991) Int. J. Cancer 49, 329–334 and Saito, S., Orikasa, S., Satoh, M., Ohyama, C., Ito, A., and Takahashi, T. (1997) Jpn. J. Cancer Res. (Gann) 88, 652–659). We now report two disialogangliosides, G1 and G2, found in the RCC cell line TOS-1. G1 from TOS-1 cells was characterized as having a novel hybrid structure between ganglio-series (region I as in Structure FTI; same as the terminal structure of ganglioside GM2), and the lacto-series type 1 (region II). The characterization was based on reactivity with various monoclonal antibodies (mAbs) with defined epitope specificity, as well as monosaccharide and fatty acid component analysis, ^1H NMR spectroscopy, and electrospray ionization mass spectrometry of the intact compound. G1 showed strong reactivity with mAb RM2, raised originally against TOS-1 cells, and weak cross-reactivity with anti-GM2 mAb MK-1–8. The antigen is hereby termed GalNAc disialosyl Lc_4Cer (IV^4GalNAcIV^3NeuAcIII^6NeuAcLc_4; abbreviated GalNAcDSLc_4). G2 was identified by^1H NMR and mass spectrometry as having a structure similar to Structure FTI but without the GalNAcβ1→4 substitution and showed strong reactivity with mAb FH9 reported previously to be specific for disialosyl lacto-series type 1 (disialosyl Lc_4) having vicinal α2→3 and α2→6 sialosyl residues, an antigen associated with human colonic cancer. Clinicopathological studies indicate that expression of these disialoganglioside antigens in RCC tissue is correlated with the metastatic potential of RCC.
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| 21.
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宮里, 実 ; 有賀, 拓郎 ; 前本, 均 ; 草田, 武朗 ; 芦刈, 明日香 ; 上間, 南海子 ; 宮城, 亮太 ; 仲西, 昌太郎 ; 呉屋, 真人 ; 村山, 貞之 ; 斎藤, 誠一 ; Miyazato, Minoru ; Ariga, Takuro ; Maemoto, Hitoshi ; Kusada, Takeaki ; Ashikari, Asuka ; Uema, Namiko ; Miyagi, Ryota ; Nakanishi, Shotaro ; Goya, Masato ; Murayama, Sadayuki ; Saito, Seiichi
概要:
Background: We retrospectively evaluated the outcome of brachytherapy with permanent iodine-125 seed implantation for pr
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ostate cancer. Methods: From 2009 to2016, 150 patients（49 in low risk, 81 intermediate, 20 high），mean age 67.1 y, which were performed brachytherapy in our hospital were enrolled in this study. Results: The average prostate volume was 27.4cc, mean International Prostate Symptom Score 9 in these patients. In postplan evaluation, mean prostate V100 93.8%, D90 in brachytherapy alone 173.9Gy, combined with external beam radiation 116.8Gy, rectum V100 in brachytherapy alone 0.27cc, combined with external beam radiation 0.13cc, urethraD90 in brachytherapy alone 89.9Gy, combined with external beam radiation 64.4Gy. The biochemical progression-free survival in 5 years was 97.0% in low risk, while 96.3% in intermediate and no patients in high risk demonstrated PSA relapse during the observation. Migration of seeds was occurred in 3.3%. Adverse event was urinary retention in 2, hematuria in 2, proctitis in 2, rectal bleeding in 2, more than grade 3 was noted in only one patient. Conclusions: The outcome of brachytherapy in our hospital was safe and feasible with low side effects.
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