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論文（リポジトリ） |
論文（リポジトリ）
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Akamine, Kozue ; Mekaru, Keiko ; Gibo, Keiya ; Nagata, Chinatsu ; Nakamura, Rie ; Oishi, Sugiko ; Miyagi, Maho ; Heshiki, Chiaki ; Aoki, Yoichi
概要:
Purpose: To investigate impact of the one‐carbon metabolism (OCM) on oocyte maturity and embryo development.\nMethods: T
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his prospective study analyzed 18 women who agreed to participate. We measured the OCM biomarkers’ concentrations including Vitamin B12 (VB12), folic acid (FA), and homocysteine (Hcy) in serum and follicular fluid (FF), and assessed their correlation. We also evaluated the influence of such OCM biomarker concentrations in mono‐FF on oocyte maturation, fertilization, embryo quality, and consequent pregnancy after embryo transfers.\nResults: All biomarkers showed a high concentration variability in different follicles of each woman, but their mean levels correlated with the serum levels. Among the 106 collected oocytes, 92 were mature, 59 were fertilized, and 16 yielded good‐quality embryos. We performed 26 single embryo transfers, and 7 patients achieved clinical pregnancies. VB12 concentration (FF) was significantly lower in fertilized than unfertilized oocytes by univariate analysis. In multivariate logistic analysis, a significant correlation was found between FA concentration (FF) <14.25 ng/mL and good‐quality embryos and between Hcy concentration (FF) <4.9 nmol/mL and clinical pregnancy.\nConclusion: OCM in FF may affect fertilization, embryo quality, and clinical pregnancy.
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論文（リポジトリ） |
論文（リポジトリ）
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広瀬, 直人 ; 前田, 剛希 ; 玉城, 盛俊 ; 和田, 浩二 ; 宮城, 一菜 ; Hirose, Naoto ; Maeda, Goki ; Tamaki, Moritoshi ; Wada, Koji ; Miyagi, Kazuna
概要:
沖縄県伝統野菜の呼吸量と食品機能性に寄与する栄養成分含量を測定した。呼吸量やアスコルビン酸含有量は、ニシヨモギやボタンボウフウおよびカラシナで高かった。総ポリフェノール含有量は、ニシヨモギやボタンボウフウで高かった。カロテノイド含有量は、ニ
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シヨモギやホソバワダンおよびスイゼンジナで高かった。ヘチマは、測定したいずれの項目でも低値であった。葉酸はボタンボウフウ、カラシナ、ホソバワダンおよびヘチマで高かった。ニシヨモギとカラシナにおいて、呼吸量とポリフェノール含有量に系統間の差異があることを確認した。これらの知見は、呼吸量と栄養成分含量を指標とした鮮度保持技術の開発に加えて、系統選抜や品種育成に貢献できる。
Respiration rate and content of components in major Okinawan traditional vegetables were measured. The respiration rate and the ascorbic acid content were high in Nishiyomogi, Botanbouhuu and Karashina. The polyphenol content was high in Nishiyomogi and Botanbouhuu. The carotenoid content was high in Nishiyomogi, Hosobawadan and Suizenjina. On the other hand, Hechima showed lower in respiration rate and these contents. Folic acid content was high in Botanbouhuu, Karashina, Hosobawadan and Hechima. In Nishiyomogi and Karashina, differences in respiration rate and total polyphenol content were shown between lines. These ndings can contribute not only development of freshness preservation technology based on indexes of respiratory rate and the contents of nutritional components but also the line selection and breeding for Okinawa traditional vegetables. 論文 続きを見る |
