1.

論文(リポジトリ)

論文(リポジトリ)
Oishi, Sugiko ; Mekaru, Keiko ; Tanaka, E. Suguru ; Arai, Wataru ; Ashikawa, Kyota ; Sakuraba, Yoshiyuki ; Nishioka, Mikiko ; Nakamura, Rie ; Miyagi, Maho ; Akamine, Kozue ; Aoki, Yoichi
出版情報: Reproductive Medicine and Biology.  21  2022-01-29.  John Wiley & Sons Australia, Ltd
URL: http://hdl.handle.net/20.500.12000/0002019669
概要: Purpose: To investigate the relationship between the microbiome of the female genital tract and endometriosis. Methods: This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next-generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. Results: Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (p = 0.02). Conclusion: Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis. 続きを見る
2.

論文(リポジトリ)

論文(リポジトリ)
Akamine, Kozue ; Mekaru, Keiko ; Gibo, Keiya ; Nagata, Chinatsu ; Nakamura, Rie ; Oishi, Sugiko ; Miyagi, Maho ; Heshiki, Chiaki ; Aoki, Yoichi
出版情報: Reproductive Medicine and Biology.  2020-10-25.  Wiley
URL: http://hdl.handle.net/20.500.12000/47491
概要: Purpose: To investigate impact of the one‐carbon metabolism (OCM) on oocyte maturity and embryo development.\nMethods: T his prospective study analyzed 18 women who agreed to participate. We measured the OCM biomarkers’ concentrations including Vitamin B12 (VB12), folic acid (FA), and homocysteine (Hcy) in serum and follicular fluid (FF), and assessed their correlation. We also evaluated the influence of such OCM biomarker concentrations in mono‐FF on oocyte maturation, fertilization, embryo quality, and consequent pregnancy after embryo transfers.\nResults: All biomarkers showed a high concentration variability in different follicles of each woman, but their mean levels correlated with the serum levels. Among the 106 collected oocytes, 92 were mature, 59 were fertilized, and 16 yielded good‐quality embryos. We performed 26 single embryo transfers, and 7 patients achieved clinical pregnancies. VB12 concentration (FF) was significantly lower in fertilized than unfertilized oocytes by univariate analysis. In multivariate logistic analysis, a significant correlation was found between FA concentration (FF) <14.25 ng/mL and good‐quality embryos and between Hcy concentration (FF) <4.9 nmol/mL and clinical pregnancy.\nConclusion: OCM in FF may affect fertilization, embryo quality, and clinical pregnancy.
論文
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3.

論文(リポジトリ)

論文(リポジトリ)
大石, 杉子 ; 銘苅, 桂子 ; 仲村, 理恵 ; 宮城, 真帆 ; 赤嶺, こずえ ; 青木, 陽一 ; Oishi, Sugiko ; Mekaru, Keiko ; Nakamura, Rie ; Miyagi, Maho ; Akamine, Kozue ; Aoki, Yoichi
出版情報: 琉球医学会誌 = Ryukyu Medical Journal.  39  pp.59-64,  琉球医学会 — Ryukyu Medical Association
概要: Laparoscopic ovarian cystectomy usually does not require ovarian suturing. However,in patients receiving anticoagulant t herapy after a surgical procedure,there is a risk of increased bleeding when perioperative bridging of anticoagulation therapy with heparin is performed. This case report discusses the risk of bleeding with anticoagulation therapy. A 38-year-old,nulliparous female underwent laparoscopic ovarian cystectomy for mature cystic teratoma in the left ovary. Because she was administered warfarin for paroxysmal atrial fibrillation before,anticoagulation bridging with heparin was prescribed after hospitalization and 1 week before the surgery. We performed laparoscopic cystectomy with bipolar coagulation. Absence of bleeding from the surgical site was confirmed,and the patient had stable vital signs; therefore,we restarted anticoagulation bridging with heparin 6 hours after the surgery and warfarin approximately 27 hours after the surgery. Severe abdominal pain and hypotension were suddenly observed 4 days after the procedure; computed tomography revealed arterial bleeding from the left ovary,and laparoscopic hemostasis was performed immediately. Arterial bleeding was observed around the left proper ovarian ligament,and the internal abdominal hemorrhage volume was 2,060 ml. We obtained hemostasis by suturing the ovary. After the second surgery,warfarin was discontinued,and the patient was discharged 6 days later. Because 4 days after surgery is too late for bleeding to occur due to surgery,it was considered that the bleeding occurred due to compounded effect of warfarin and heparin . However,it is important to perform elaborate hemostasis by not only inducing coagulation but also including suturing the ovary. Moreover,careful follow-up is required in patients who undergo laparoscopic ovarian cystectomy and receive anticoagulant therapy.
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4.

学位論文(リポジトリ)

学位
大石, 杉子 ; Oishi, Sugiko
出版情報: Reproductive Medicine and Biology.  21  琉球大学 — University of the Ryukyus
URL: http://hdl.handle.net/20.500.12000/0002018034
概要: Purpose: To investigate the relationship between the microbiome of the female genital tract and endometriosis. Methods: This prospective cohort study included 36 women who underwent laparoscopic surgery for ovarian tumor from July 2019 to April 2020. Of them, 18 had endometriosis, and 18 did not have endometriosis. Vaginal secretions, endometrial fluid, peritoneal fluid, and ovarian cystic fluid were collected during surgery. Next-generation sequencing of bacterial 16S rRNA was performed to characterize the microbiome. Results: Specific microbiomes were not detected in either peritoneal fluid or ovarian cystic fluid regardless of the presence or absence of endometriosis and the type of cyst. When the cutoff value of infectious bacterial abundance in the vagina was set as 64.3%, there were many cases more than a cutoff value in the endometriosis group significantly (p = 0.01). When the cutoff value of infectious bacterial abundance in the endometrium was set as 18.6%, there were many cases more than a cutoff level in the endometriosis cases significantly (p = 0.02). Conclusion: Peritoneal fluid and ovarian cystic fluid are almost sterile, although dysbiosis may occur in the vaginal and endometrial microbiome in women with endometriosis. 続きを見る